mIPSC detection in dissociated hippocampal neuron culture.

Hannah K Warming
Last updated on Mar 27, 2021 1 min read 👏

Model Abstract:

A model for detection of GABA(A) mIPSCs trained on data from DIV20-22 high-density hippocampal neurons, cultured from postnatal day 0 C57 mouse. Cells were treated with vehicle, haemolysate or blood plasma products for 1 week before recording.

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Author(s):

Author

Hannah K Warming

Affiliation

University of Southampton

Contact

hkw1g14@soton.ac.uk

Twitter

@WarmingHannah

Experimental Methodology:

Preparation

Dissociated cell culture

Recording Type

mIPSC

Cell Type

Hippocampal neurons

Recording Temperature (celsius)

25

Intracellular Solution

Intracellular solution containing in mM: CsCl 130, NaCl 5, BAPTA 5, HEPES 10, QX-314 5, GTP-NaCl 0.3, ATP-Mg 4, Adjusted to pH 7.3 with CsOH.

Extracellular Solution

Standard aCSF containing in mM: NaCl 126, CaCl2 2, D-Glucose 10, MgSO4 2, KCl 3, NaHPO4 1.25, NaHCO3 and bubbled with carbogen (95% carbon dioxide/ 5% Oxygen). Drugs added for recordings: 500nM TTX and 10µM CNQX.

NA

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Dissociated cell culture mIPSC